Application value of tissue tuberculosis antigen combined with Xpert MTB/RIF detection in differential diagnoses of intestinal tuberculosis and Crohn’s disease

Abstract Background The purpose of this study was to examine the value Xpert MTB/RIF assay and detection additional Mycobacterium tuberculosis complex (MTBC) species antigens from intestinal tissue samples in differentiating (ITB) Crohn’s disease (CD). Methods Several clinical specimens obtained by either endoscopic biopsy or surgical excision were used for mycobacteriologic solid cultures,Xpert assays, immunohistochemistry, histological examinations. Four (38KDa, ESAT-6, MPT64, Ag85 complex) MTBC detected immunohistochemical analysis. Results included 42 patients with ITB 46 CD. Perianal lesions longitudinal ulcers more common CD, while caseating granuloma annular ITB. positive rate tissues 33.33%, which significantly higher than that CD using acid-fast staining smears. It also culture, but difference not statistically significant. MPT64 expression 40.48%, observed sensitivity parallelly combined protein diagnosing 50.0%. Conclusions is a rapid useful method establishing an early diagnosis antigen has definitive differential ofITB combination these two methods can improve sensitivity.